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Research paper topic: Biotin - 873 words
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Biotin Biotin is important for healthy hair and skin. 100 mg of biotin may prevent hair loss in some men. Biotin helps to relieve muscle pain. It promotes healthy nerve tissue, bone marrow and sweat glands. It also relieves seborrheic dermatitis in infants.
Biotin works with folic acid and vitamin B12 to break down fats, protein, and carbohydrates. Biotin is found in most foods and also manufactured by bacteria in the intestinal tract. Most biotin deficiencies are associated with the consumption of raw egg whites which contain avidin. Avidin binds with biotin to prevent its absorption into the blood. Cooking the egg whites deactivates avidin.
Biotin is non-toxic and probably not required in supplement form. Although biotin deficiencies are rare, they can occur when people have malabsorption problems. Some research indicates that male pattern baldness may respond to supplemented biotin, albeit in a very marginal fashion. Biotin is found in cooked egg yolks, salt-water fish, beef and poultry, milk, cheese, soy products and whole grain breads. If athletes are eating raw egg whites, extra biotin must be consumed. Reagent for enzymatic incorporation of biotin into nucleic acids. Biotin-21-dUTP is a dTTP analog with biotin covalently attached to the pyrimidine ring through a 21-atom spacer arm. It can be incorporated by nick translation, mixed primer labeling, 3'-end-labeling, or PCR. Biotin-21-dUTP has a 21-atom spacer arm, the longest spacer available, which reduces steric hindrance in subsequent detection using streptavidin-conjugated enzymes. 100 l is sufficient for 50 nick translation reactions.
Biotin-21-dUTP is provided with a complete User Manual (PT1464-1). Product Size Cat. # Biotin-21-dUTP (0.5 mM) 100 l 5021-1 Biotin-21-dUTP (10 mM) 100 l 5021-3 Form 100 l of 0.5 mM solution in 50 mM Tris-HCl (pH 7.5) or 100 l of 10 mM solution in 50 mM Tris-HCl (pH 7.5) Storage Conditions: -20C Biotin plays a key role in turning fat, carbohydrates and amino acids into fuel for the body. It also helps strengthen hair and nails. Food Sources 3/4 cup of soybeans or 1/2 cup of oatmeal = 30 mcg (Adequate Intake for adult male) Brewer's yeast, liver, oatmeal, soybeans, eggs, salmon, milk, mushrooms, halibut, bananas, peanut butter, cantaloupes, cottage cheese.* *Foods have been placed in descending order from best to good source. Deficiency Signs, Symptoms and Those at Risk Signs of deficiency in infants are skin rash and/or scaly inflammation of the skin.
Signs of deficiency in adults are dry, scaly skin; nausea; fatigue; depression; and hair loss. Infants under six months born with very low levels of biotinidase (the enzyme that activates biotin) are at risk for deficiency. Adults on long-term anti-convulsant drugs, which can inhibit biotin uptake in the intestines, are at risk for deficiency. Dosage Information Age Adequate Intake Under 6 months 5 mcg 6-12 months 6 mcg 1-3 years 8 mcg 4-8 years 12 mcg 9-13 years 20 mcg 14-18 years 25 mcg Males 19+ 30 mcg Females 19+ 30 mcg Pregnant 30 mcg Lactating (1st 12 mths) 35 mcg Toxicity/Possible Side Effects To date, no cases of biotin toxicity have been reported. [INDEX] 1-85 Deficiencies of Vitamins B1 (Thiamine), B2 (Riboflavin), B3 (Niacin) and the cofactor (helper substance) Biotin reduce immune resistance markedly. If B5 (Pantothenic Acid) or B6 (Pyridoxine) are deficient, resistance drops almost to zero.
A good B Complex is necessary to compliment Vitamin C when fighting infections. 1-144 Biotin is essential to the metabolism of carbohydrates and fats. It has no known toxicity. Any supplemental form is as good as another. The best natural sources of Biotin are soybeans and brown rice. Raw egg white contains a substance called avidin, which prevents Biotin from being absorbed into the body. Biotin can help with hair loss only when used as part of a complete vitamin and mineral supplement.
BIOTIN A coenzyme. In 1916 it was discovered there was a detrimental effect in eating raw eggs.* susceptible to strong acid and alkaline, UV stable in heat, visible light and air the D-isomer id its active form Absorption absorbed in the small intestine mainly by active transport (some passive absorption and possibly some bacteria synthesis) storage of biotin occurs in the liver, kidney and adrenals * inhibited by avidin which is found in raw egg whites Increased requirements alcohol, coffee, raw egg whites pregnancy, lactation aging athletes dermatitis Excretion In the urine mainly. Synergistic nutrients increases the synthesis of B12 convert tryptophan to B3 with B2, B3, B6, A in skin function with B5 in hair pigmentation Mg and Phosphorus are needed to convert biotin into its active form needed to activate B5, B9, B12 Zn metabolism Functions 1. carboxylation and decarboxylation reactions in protein metabolism 2. carbohydrate metabolism 3. synthesise and secrete insulin 4. glycogen synthesis 5. fat metabolism 6.
antibody and haemoglobin production 7. pancreatic amylase production 8. maintain health of hair, skin, sebaceous glands, bone marrow, sex glands 9. can help cholesterol plaques in blood vessels Deficiency Symptoms GIT - nausea, vomiting, anorexia Skin grey skin (although they are not dying), alopecia (hair loss), scaly dermatitis, skin dryness increased lactic acid levels increased blood cholesterol hypoglycaemia greying hair Reproductive abnormalities due to mothers deficiency - cleft palate, micromelia (abnormally small limbs) Therapeutic uses for the deficiencies above candida (biotin decreases levels of Candida) atherosclerosis acne leg cramps diabetes burns and scalds Toxicity None known. Dose RDA 200 mcg Therapeutic 150-600 mcg Sources Most animal and plant tissues, eg.
egg yolk, liver, nuts, soya beans, brown rice, Royal jelly, bee pollen, Brewers yeast.
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